Si-IDA-Ni™ Magnetic Beads Kit for Purification of His-Tagged ProteinsSi-IDA-Ni magnetic beads are designed for purification of histidine tag (His-tag) proteins purification. Unlike the
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conventional metal-chelated agarose or dextran pre-packed column these Si-IDA-Ni beads are made of newly-designed functional material with paramagnetic properties. Ni-IDA beads have much higher binding capacity than Ni-NTA beads and require a lower imidazole concentration. The separation and purification of the (6x) His tagged proteins from cell lysates is accomplished via magnetic field force. Through this simple, one-step purification procedure, high purity of both N- or C-terminal His-fusion proteins can be obtained. Si-IDA-Ni magnetic beads can be regenerated and reused multiple times (see below). 
Purification of His-fusion proteins by Si-Mag-Ni beads |
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| Lane 1: M.W. marker | |
| Lane 2: 10 uL of purified protein X | |
| Lane 3: 4 uL of purified protein Y | |
| Lane 4: 4 uL of purified protein Z | |
| Lane 5: 10 uL of purified protein X (different vendor) | |
| Lane 6: 4 uL of purified protein Y (different vendor) | |
| Lane 7: 4 uL of purified protein Z (different vendor) | |
Regeneration of Si-Mag-Ni beads |
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| Lane 1: M.W. marker | |
| Lane 2: Crude cell lysate | |
| Lane 3: Purified protein X by fresh beads | |
| Lane 4: Purified protein X by beads regenerated once | |
| Lane 5: Purified protein X by beads regenerated twice | |
| Lane 6: Purified protein X by beads regenerated 3 times | |
| Lane 7: Purified protein X by beads regenerated 4 times | |
| Lane 8: Purified protein X by beads regenerated 5 times | |
| Lane 9: Purified protein X by beads regenerated 6 times | |
| Lane 10: Purified protein X by beads regenerated 7 times | |
| Lane 11: Purified protein X by by beads regenerated 8 times | |